Differential regulation of dynein and kinesin motor proteins by tau.
نویسندگان
چکیده
Dynein and kinesin motor proteins transport cellular cargoes toward opposite ends of microtubule tracks. In neurons, microtubules are abundantly decorated with microtubule-associated proteins (MAPs) such as tau. Motor proteins thus encounter MAPs frequently along their path. To determine the effects of tau on dynein and kinesin motility, we conducted single-molecule studies of motor proteins moving along tau-decorated microtubules. Dynein tended to reverse direction, whereas kinesin tended to detach at patches of bound tau. Kinesin was inhibited at about a tenth of the tau concentration that inhibited dynein, and the microtubule-binding domain of tau was sufficient to inhibit motor activity. The differential modulation of dynein and kinesin motility suggests that MAPs can spatially regulate the balance of microtubule-dependent axonal transport.
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www.sciencemag.org (this information is current as of March 19, 2008 ): The following resources related to this article are available online at http://www.sciencemag.org/cgi/content/full/319/5866/1086 version of this article at: including high-resolution figures, can be found in the online Updated information and services, http://www.sciencemag.org/cgi/content/full/1152993/DC1 can be found at: ...
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ورودعنوان ژورنال:
- Science
دوره 319 5866 شماره
صفحات -
تاریخ انتشار 2008